The Role of β-Catenin Pathway on DDP Resistance in Human Lung Cancer A549 Cell

Objective To establish a cisplatin(DDP) resistance A549 cell line and to explore the mechanisms of multidrug resistance in human lung cancer.Methods The human cancer A549 cells were exposed in gradually increasing or interval high dose of DDP.MTT assay was used to detect the cytotoxic activity of DDP against A549 and A549/DDP cells.Cell numbers were calculated to draw growth curve.The protein expressions of Akt,p-Akt,GSK-3β,p-GSK-3β and β-Catenin were determined by western blotting analysis in A549 and A549/DDP cells.Results DDP resistance cell line A549/DDP was established by DDP continuous inducing.The IC50 values of DDP against A549 and A549/DDP cells were(1.37±0.09) and(11.63±0.74) μmol/L by MTT assay respectively.The cells growth curve showed that cell proliferation had no significant difference between A549 and A549/DDP cells.Western blotting analysis showed that phospho-Akt was up-regulated in A549/DDP cells,which elevated the phosphorylation of GSK-3β at serine 9.Compared to A549 cells,the up-regulation of β-Catenin protein was found in A549/DDP cells.Conclusions A549/DDP showed resistant to DDP and was a reliable multidrug resistance cell model.The β-Catenin pathway played an important role in the resistant phenotype of A549/DDP cell line.