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2011•Research and Practice on Chinese MedicinesRequires access

Content Determination of Honokiol in QianBeis Magnolia officinalis by HPLC

Xiaodong Wang

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Abstract

Objective To establish a method for the content determination of honokiol in QianBeis Magnolia officinalis by HPLC.Methods A Diamonsil C18 column was used with mobile phase of methanol-water(78 : 22),the detection wavelength was 294nm,the flow rate was 1.0 mL/min.Results The calibration curve showed a good linearity with the range of 0.627 2~1.164 8 μg,r=0.999 4.The average recovery was 102.12% and RSD was 1.3%.Conclusion The method is simple,sensitive and accurate.

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What this paper is about

Objective To establish a method for the content determination of honokiol in QianBeis Magnolia officinalis by HPLC.Methods A Diamonsil C18 column was used with mobile phase of methanol-water(78 : 22),the detection wavelength was 294nm,the flow rate was 1.0 mL/min.Results The calibration curve showed a good linearity with the range of 0.627 2~1.164 8 μg,r=0.999 4.The average recovery was 102.12% and RSD was 1.3%.Conclusion The method is simple,sensitive and accurate.

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Available abstract

Objective To establish a method for the content determination of honokiol in QianBeis Magnolia officinalis by HPLC.Methods A Diamonsil C18 column was used with mobile phase of methanol-water(78 : 22),the detection wavelength was 294nm,the flow rate was 1.0 mL/min.Results The calibration curve showed a good linearity with the range of 0.627 2~1.164 8 μg,r=0.999 4.The average recovery was 102.12% and RSD was 1.3%.Conclusion The method is simple,sensitive and accurate.

Key concepts: Honokiol, Chromatography, Magnolia officinalis, Magnolol, Calibration curve, High-performance liquid chromatography, Chemistry, Recovery rate

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