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2014•Unpublished venueRequires access

The RP-HPLC Method for Determination of Different Old Purple Oil Honokiol and Magnolol in Magnolia Bark

LI Hongyin

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Abstract

The HPLC is used to determine the content of purple Magnolia officinalis medicinal herbs and Magnolol and honokiol,content. ODS2C18( 200 mm x 4. 6 mm,5 μm) chromatographic column is a dopted,and methanol ∶ water = 75 ∶ 25( V /V) is taken as mobile phase. Detection wavelength of 294 nm,velocity: 1. 0 ml /min,column temperature 25℃. The results of Magnolol,the average recovery was98. 10%,RSD = 0. 42%. Honokiol the average recovery was 99. 86%,RSD = 0. 86%. The method has good repeatability,high precision,good linearity,rapidity and high accuracy. and provides a scientific basis for Magnolia officinalis medicinal materials quality evaluation,variety breeding and content control.

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What this paper is about

The HPLC is used to determine the content of purple Magnolia officinalis medicinal herbs and Magnolol and honokiol,content. ODS2C18( 200 mm x 4. 6 mm,5 μm) chromatographic column is a dopted,and methanol ∶ water = 75 ∶ 25( V /V) is taken as mobile phase. Detection wavelength of 294 nm,velocity: 1. 0 ml /min,column temperature 25℃. The results of Magnolol,the average recovery was98. 10%,RSD = 0. 42%. Honokiol the average recovery was 99. 86%,RSD = 0. 86%. The method has good repeatability,high precision,good linearity,rapidity and high accuracy. and provides a scientific basis for Magnolia officinalis medicinal materials quality evaluation,variety breeding and content control.

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Available abstract

The HPLC is used to determine the content of purple Magnolia officinalis medicinal herbs and Magnolol and honokiol,content. ODS2C18( 200 mm x 4. 6 mm,5 μm) chromatographic column is a dopted,and methanol ∶ water = 75 ∶ 25( V /V) is taken as mobile phase. Detection wavelength of 294 nm,velocity: 1. 0 ml /min,column temperature 25℃. The results of Magnolol,the average recovery was98. 10%,RSD = 0. 42%. Honokiol the average recovery was 99. 86%,RSD = 0. 86%. The method has good repeatability,high precision,good linearity,rapidity and high accuracy. and provides a scientific basis for Magnolia officinalis medicinal materials quality evaluation,variety breeding and content control.

Key concepts: Magnolol, Honokiol, Magnolia officinalis, Chromatography, Repeatability, High-performance liquid chromatography, Chemistry, Bark (sound)

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The RP-HPLC Method for Determination of Different Old Purple Oil Honokiol and Magnolol in Magnolia Bark — Research Paper | ScholarLens