Effect of Maltose on Efficiency of Anther Culture of Rice

The effect of different sugars (150 mmol/L for each) on callus induction in rice anther culture was innitially investigated by using three indica cultivars. Maltose resulted in greater microspore callus induction than sucrose and other sugars. Further experiments with 7 indiea, 2 japonica cultivars and several hybrids indicated that mean percentages of callus induction and then green plantlet regeneration were 54. 8% and 9. 70%, respectively, on maltose medium, which were significantly higher than those on sucrose medium. The strategy of raising the efficiency of indica rice anther culture and suitable calculative formula for callus induction rate are discussed.