Inhibition of YH-16 on growth of both Hela cells and Hela implanted tumor in nude mice

[Objective] To evaluate the inhibitory effect of YH-16(rhEndostatin) on the growth of cervix cancer cell lines Hela and Hela implanted tumor in nude mice. [Methods] The effect of Endostatin on Hela cell proliferation was studied by means of MTT. The cell apoptosis was detected by transmission electron microscope and cell flow cytometry. The Hela cells were subcutaneously inoculated into nude mice. The therapeutic efficacy of different doses YH-16 (0, 0.4, 0.75 and 1.5 mg/kg) on bearing cancer mice was detected. The cell apoptosis of implanted tumor was detected by TUNEL stanning. [Results] YH-16 inhibited Hela cells proliferation (P 0.01) and induced cell apoptosis. YH-16 significantly suppressed the growth of the implanted rumor in nude mice(P 0.05). The MVD in mice treated with YH-16 15mg/kg was much lower than other groups(P 0.05). [Conclusions] Endostatin had inhibitory effects on the growth of both Hela cell and implanted tu mors in nude mice.