The Value of chemiluminescent immunoassay for measurement of anti-cyclic citrullinated peptide antibody in the diagnosis of rheumatoid arthritis

Objective To explore the value of chemiluminescent immunoassay for measurement of anti- cyclic citrullinated peptide( anti- CCP) antibody in the diagnosis of rheumatoid arthritis. Methods Chemiluminescent immunoassay was performed to detect anti- CCP antibody in 85 patients with rheumatoid arthritis( RA),57 patients with other autoimmune diseases( 10 with systemic lupus erythematosus,11 patients with ankylosing spondylitis,19 patients with connective tissue diseases,17 patients with sjogren syndrome). In addition,serum rheumatoid factor( RF),immunoglobulin G( Ig G) and C reactive protein( CRP) were detected to for a joint analysis. Results Compared to patients with other autoimmune diseases,the level and the positive rate of anti- CCP antibody and RF in patients with RA were significantly higher,the difference was statistically significant( P 0. 01); With respect to that of Ig G and CRP,the difference were not statistically significant between them( P 0. 05). The level of CRP in RA patients with positive anti- CCP antibody was significantly higher than those of RA patients with negative anti- CCP antibody( P 0. 01). The sensitivity and specificity of anti- CCP antibody detected by chemiluminescent immunoassay in the diagnosis of RA were 77. 6% and 87. 7%,respectively. Conclusion Anti- CCP antibody detected by chemiluminescent immunoassay has good specificity,but little lower on sensitivity for RA. The combination of anti- CCP antibody and RF may offer clinical benefits for diagnosis of RA and reduce the rate of misdiagnosis.