Buzhong Yiqi Decoction on Traumatized Rat Immune T Cell Subgroup Effects

Objective:Using a rat model of blunt trauma,observed 7 days after the trauma of Buzhong Yiqi Decoction after application of the immune regulation effects.Methods:Healthy adult SD rats were randomly divided into three groups,normal control group(A),control group(B) trauma,posttraumatic Buzhong Yiqi Decoction group(C).B group and C group made bilateral femur closed fracture trauma model,and 1h after injury to the A group,B group by gastric perfusion with saline,to group C by gastric perfusion of Buzhong Yiqi Decoction,six hours,one day,three days,five days or seven days after the trauma,get 1ml determination from femoral vein blood immune indexes in T cell phenotype and CD4,CD8 and the content of CD3.Results:After trauma in rats in B group and C group at all time periods of six hours,one days CD3+T,CD4+T cell number than trauma significantly decreased,the number of T cells increased significantly in CD8+,CD4+T /CD8+T decreased significantly(P0.01),three days,five days or seven days after the trauma,blood T lymphocyte of group C group B CD3+T,CD4+T cell number get increased blood T lymphocyte group B CD3+T,CD4+T cell number increased,CD8+T cell number decreased,CD4+T/CD8+T increased compared with the B group had statistical significance(P0.05).CD3+T,CD4+T cell numbers increases more in seven days than in six hours,one day,three days or five days CD3+T,CD4+T cell numbers have increased,CD8+T cell numbers have declined,CD4+T/CD8+T has risen,normal control group without change.Conclusion:Trauma in rats after T lymphocyte subsets in numerical decline,presence of cellular immunosuppression,but with time after injury migration cell immune function there are signs of recovery,the application of Buzhong Yiqi Decoction could obviously increase the CD4+T,CD4+T/CD8+T cell number,ratio of T cell increased,and to a certain extent reduced T cell surface CD8+ the expression of T cell.