Reversal of Resistance to Adriamycin in Human Breast Cancer Cell MCF-7/ADM by Genistein

Objective To explore the effects of genistein single and combined adriamycin on drug resistance of human breast cancer cells MCF-7/ADM.Methods The MCF-7/ADM cells were co-cultured with different concentrations of genistein(8,15,30,60 μg/mL) and adriamycin for 48 h and 72 h in vitro,respectively.The inhibitory rates(IR) of MCF-7/ADM cells were detected by MTT assay and the resistant index and reversal index were calculated.Results The resistant index of MCF-7/ADM were 117.15 times at 48 h,and 54.42 times at 72 h than those of MCF-7/S.The inhibitory rates of MCF-7/ADM cells were positively correlated with time and dosage.The inhibitory rates of cells treated with 25 μg/mL genistein combined different concentrations of adriamycin were significantly higher than those of single administered(P0.01),and the reversal index was 7.53 times.Conclusion Genistein could inhibit the growth and reversal the resistance of breast cancer MCF-7/ADM cells,the combination of genistein and adriamycin could produce a synergistic effect.