Effects of Yishentongluo decotion on the expression of NF-κB and MMP-2 on experimental membranous nephropathy

Objective To observe the effects of Yishentongluo decotion on the expression of NF-κB and MMP-2 on experimental membranous nephropathy (MN).Methods SD rats were randomly divided into control,model,benazepril and Chinese herb groups (10 rats each group),and given with the medicine respectively.At the ninth week,24 h urine protein quant (UPro),TP,ALB were determined,electron microscope and immunofluorescence methods were used to detect the expressions of NF-κB and MMP-2.Results Compared with those of normal group,the 24 h urinary protein quantitative analysis and the serum level of TC and TG of model group were sharply increased;the serum TP and ALB levels were significantly lower,nephritic injury incurred.The expression of NF-κB,MMP-2 in model group increased(P0.05).Compared with those of model group,the 24 h urinary protein quantitative and the serum level of TC and TG of each treatment group were sharply decreased;the serum TP and ALB of were significantly higher;nephritic injury was lighter.The expression of NF-κB,MMP-2 in model group increased as compared to those of benazepril and Chinese herb groups(P0.05).There was no markable difference between the Benazepril and Chinese herb groups(P0.05).Conclusions Yishentongluo decotion can reduce rat's proteinuria,increase plasma protein,improve blood rheology to protect renal function.Yishentongluo decotion can depress NF-κB and MMP-2 expression in nephridial tissue.