Haemophilus Isolated from Respiratory Tract Infections and Their Drug Resistance from 2010 to 2012

Objective To investigate the distribution and drug resistance of Haemophilus isolated from respiratory tract in our hospital from 2010 to 2012,and guide the antibiotic practice. Methods Haemophilus was isolated by Colombia chocolate medium,preliminarily identified by satellite testing and confirmed by the NH identification card. The disc agar diffusion method( Kirby-Bauer) was employed for susceptibility testing and detection of β-lactamase. Results From 2010 to 2012,we isolated 394 Haemophilus,which included 126( 31. 2%) Haemophilus influenzae and 268( 68. 8%) Haemophilus parainfluenzae. The production of beta-lactamase of Haemophilus was 40. 8%. The resistance rate of Haemophilus influenzae to cefuroxime sodium,ciprofloxacin,ampicillin and cotrimoxazole was very high and increased year by year. The same as Haemophilus influenzae,the resistance rate of the Haemophilus parainfluenzae to cefuroximesodium,ciprofloxacin,levofloxacin,ampicillin and cotrimoxazole was also markedly high and increased year by year. However,the resistance rates of the two types of Haemophilus to third and fourth generation cephalosporins,penicillins with beta-lactamase inhibitors drug and carbapenem was significantly low. Conclusion The positive rate of Haemophilus influenzae was lower than that of Haemophilus parainfluenzae. The third and fourth generation cephalosporins and penicillins with beta-lactamase inhibitors drug were the first choice in the treatment of infections caused by this type of bacteria. The clinical microbiology room should play more attentions on the conventional isolation,culture and sensitivity testing on Haemophilus isolated from respiratory tract infection,and provides the basis for rational using of antibiotics and thus reduces the resistance of Haemophilus.