Preliminary observation on the effect of incubation time on the toxin production of Fusarium oxysporum

Objective The aim of the study is to explore the optimum condition to cultivate the Fusarium oxysporum and produce T-2 fungal toxin.Methods The fungi were isolated and purified after the cultivation of 8 days,second filial generation sporus were inoculated and cultivated on sterile corn(pH 7.0~7.2)with different time(2w,4w,6w),the temperature was 15 ℃.Results The contents of T-2 fungal toxin were detected with high performance liquid chromatogram(HPLC).The result shows that the cultivated time is the important factor and the content of T-2 fungal toxin is different,6w≥4w≥2w.Conclusion The cultivated time is the important factor of the content of T-2 fungal toxin.