ChAT change in C_4 spinal cord tissue after C_4 cervical plexus transposition for repairing brachial plexus avulsion

Objective To observe after ChAT changes in C4 spinal cord tissue C4 transposition to repair brachial plex-us avulsion in rats.Methods 30 Wistar rats were randomly divided into group A,B and C,10 each group.Group A and B were established a brachial plexus avulsion model.Group A was the experimental group,C4 transposition were anderwent for repairing brachial plexus avulsion;group B was control group,C4 and C6 nerve root was cut and no anastomosis at the intervertebral foramen;group C was normal control group,without operation.After 3 months,the nerve 0.5 cm was got be-tween C4 and C6 anastomotic in group A,0.5 cm in the stump of C6 in group B and 0.5 cm in C6 in group C were stained with HE and then observed;at the same time,C4 spinal cord tissues were stained by immunohistochemical ABC method,the number of ChAT spinal anterior horn motor neuron immunoreactive positive neurons was calculated.Results ①Right forelimb flexion of group A was normal,triceps brachii-two was no atrophy;right forelimb flexion of group B animal was not a straight,deformity,and triceps brachii-two belly of the muscle was atrophy;group C was normal.②After HE staining,there was a lot of regenerating nerve fibers and myelin,uniform,dense,epicardial continuous in group A,and nerve fiber was arranged neatly;there were small nerve fibers which was disordered and many foreign objects in group B;the nerve fi-ber is uniform,dense,neat in group C.③The ChAT immunoreactive motoneurons numbers of C4 spinal cord anterior horn of group A,B and C were 23.27 ± 3.35,19.53 ± 2.97,25.53 ± 3.42,respectively.That were different in group A,C compared with group B(all P 0.05),and group A was similar to group C(P 0.05).Conclusions After C4 transfering for repairing brachial plexus,ChAT expression in C4 spinal cord tissue is up,which may be one of the reasons that C4