Insulin Resistance and Hypertension Rat Model Induced by Fructose-rich and Its Features

Objective The aim of the study was to create a rat model(Fructose Fed Rats, FFR)of insulin resistance (IR) and hypertension ,and to investigate its features. Methods Six week old male Sprague Dawley rats were fed with either normal rat chow groups(60% vegetable starch, 11% fat and 29% protein, n =35) or fructose rich diet groups(66% fructose, 12% fat and 22% protein, n =35).In all rats, The euglycemic hyperinsulinemic glucose clamp(GC)technique was performed to estimate insulin sensitivity. Blood pressure was elevated with tail cuff method. Results It was showed that blood pressure of the FFR groups was significantly increased compared with control groups. The average rate of glucose clamp,as a measure of insulin sensitivity (M value), and weight were significantly lower in FFR compared with control ( P =0.012-0.002) after 4 weeks or 8 weeks. TG was significantly decreased in the control groups compared with FFR groups ( P =0.036).However, FFA and FINS were the same as TG,with no significant diffrence( P =0.066,0.074). Conclusion This rat model is characterized by typical insulin resistance and hypertension. It may provide a advantageous tool for investigations of insulin resistance and hypertension.