Mixed fresh serum of patients as a temporary calibrator for calibrate of non-matchinganalytic system

Objective Take mixed fresh serum from the patients as a calibration fluid detection system for non-matching 7170A automatic biochemical analyzer in analysing the source.Methods With Bayer reagents and procedures,calibration materials,quality control,and Bayer 2400 automatic biochemical analyzer detection system,the standard value of 7170A/Lidemen reagents was detected directly in accordance with the standard of Roche Calibrator cfas;and then mixed with fresh serum of patients as a temporary calibrator,Roche Calibrator cfas accuracy by the standard system after the transfer,the Roche Calibrator cfas value was converted to the marked correction Hitachi 7170A/Lidemen reagents,Then the accuracy was transmitted to the fresh mixed patient sera from the standard system.The indicative value of the Roche Calibrator cfas was transferred into the actual value in the 7170A/Lidemen system.UREA、ALT,CK,Glu,ALB,Cr,UA,TBIL,TC in 40 fresh sera were measured by the two analytic systems mentioned above,and the results of linear regression were analysed.Results After the accuracy delivered by Roche cfas calibrator value of goods marked by the standard system,and when the direct correction of 7170A /reagent systems was finished,the mean and standard indicators of the mean relative bias system were 1.65%,2.61%,3.11%,2.05%,1.30%,3.45%,2.02%,3.9%、and 2.94% respectively;the relative bias were all less than 1/2CLIA'88.Meanwhile,the linear regressions were good.Conclusion The mixed fresh serum of patients as a temporary calibration goods,after the transimission of the accuracy by the standard system,can be used for correcting non-matched detection system.