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2009•Journal of Chongqing Institute of TechnologyRequires access

Research on Induced Apoptosis of VacA in HeLa cell

Jiang He

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Abstract

This study is to confirm that VacA of helicobacter pylori induce Hela cells to apoptosis.MTT is applied to assay the inhibiting action of VacA.The apoptosis rates are measured with flow cytometry.The growth of Hela cells is effectively inhibited by VacA.A clear close and time dependent correlation between VacA concentration and the degree of apoptosis induction is observed with up to 56.19% apoptosis cells after 48h of incubation with 5mg/ml VacA.The cells assumed typical cell apoptosis configuration.VacA induces the apoptosis of the cells.

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What this paper is about

This study is to confirm that VacA of helicobacter pylori induce Hela cells to apoptosis.MTT is applied to assay the inhibiting action of VacA.The apoptosis rates are measured with flow cytometry.The growth of Hela cells is effectively inhibited by VacA.A clear close and time dependent correlation between VacA concentration and the degree of apoptosis induction is observed with up to 56.19% apoptosis cells after 48h of incubation with 5mg/ml VacA.The cells assumed typical cell apoptosis configuration.VacA induces the apoptosis of the cells.

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Available abstract

This study is to confirm that VacA of helicobacter pylori induce Hela cells to apoptosis.MTT is applied to assay the inhibiting action of VacA.The apoptosis rates are measured with flow cytometry.The growth of Hela cells is effectively inhibited by VacA.A clear close and time dependent correlation between VacA concentration and the degree of apoptosis induction is observed with up to 56.19% apoptosis cells after 48h of incubation with 5mg/ml VacA.The cells assumed typical cell apoptosis configuration.VacA induces the apoptosis of the cells.

Key concepts: HeLa, Apoptosis, Flow cytometry, MTT assay, Chemistry, Molecular biology, Cell, Cell biology

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