Study on aqueous two-phase extraction of L-phenylalanine by polyethylene glycol/salt system

An aqueous two - phase system of polyethylene glycol(PEG)/salt with the behavior of forming aqueous two - phase and the distribution rules of L - phenylalanine in the aqueous two - phase system were studied. Effects of the molecular weight of polyethylene glycol,the mass fraction of polyethylene glycol,the types and concentrations of salts,the initial concentration of L - phenylalanine and pH value were investigated. When the mass fraction of PEG1000 was 27%,the mass concentration of K2HPO4 was 0. 15g/mL,the initial concentration of L - phenylalanine was 10g/L and pH value was about 8. 5,the highest extraction yield of L - phenylalanine could reach 99. 5%,partition coefficient of L - phenylalanine could reach 186. 5.