Experimental study of inhabitation of lower concentration of paclitaxel to the proliferation of SPCA-1 cel

Objective To study the effect of lower concentration paclitaxel on the proliferation,cell cycle,apoptosis and mitosis of non-small cell SPCA-1.Methods SPCA-1 cells were treated by different concentration of paclitaxel for 16 hours,paclitaxel containing medium was replaced by drug free medium.After additional 56 hours,cells were counted and the growth inhibition rates were calculated.SPCA-1 cells were treated by 0,1.5,3 and 6 nmol/L paclitaxel with or without 10 μmol/L SP600125 for 16 hours,the cell cycle and apoptosis were determined by flow cytometry.Results Lower concentration of paclitaxel could inhibit the proliferation SPCA-1 cells significantly.The IC50,IC70 and IC90 were 1.75、3.25 and 4.50 nmol/L respectively.The apoptosis rate induced by 0,1.5,3 and 6 nmol/L were(0.5±0.36)%,(17.0±0.55)%,(27.0±0.63)%,(21.0±3.02)% and(22.7±1.68)%,(19.4±1.43)%,(26.2±0.97)%,(42.6±3.36%)were blocked at G2/M phase by the same concentration of paclitaxel.The apoptosis induced by paclitaxel was reduced significantly by 10 μmol/L SP600125,a JNK inhibitor.Conclusion Lower concentration of paclitaxel inhibited SPCA-1 cell growth by apoptosis induction and mitosis blocking,and JNK inhibitor could reduce paclitaxel-induced apoptosis.