2009•Shanxi Yike Daxue xuebaoRequires access
Effect of vitamin K3 on apoptosis of human bladder cancer cell line T24
Junsheng Liu
Open publisher page 0 citations
Abstract
Objective To explore the inducing effect of vitamin K3 on apoptosis of human bladder cancer cell line T24.MethodsT24 cells were cultured in the presence of different concentrations of VK3(0,2,5,10,20 μmol/L).The inhibitory rates of VK3 were detected by MTT colorimetry,and apoptosis was observed using flow cytometry.ResultsAfter T24 cells were cultured with VK3 for 48 h at concentrations of 0,2,5,10,20 μmol/L,the inhibitory rates of VK3 were(5.80±0.038)%,(13.30±0.036)%,(26.55±0.032)%,(42.58±0.028)% and(65.35±0.034)%,respectively,and the apoptotic rates were(2.86±0.4)%,(5.16±0.7)%,(10.85±1.1)%,(23.01±0.8)% and(75.80±1.2)%,respectively.Cell growth significantly decreased in the presence of 10 μmol/L,20 μmol/LVK3(P0.05).ConclusionVK3 at the concentrations of 10-20 μmol/L could obviously inhibit the proliferation of T24 cells and induce apoptosis after 48 h culture in vitro.