Tiletamine Induced c-fos Oncogene Expression within Central Nervous System of Rats

To investigate the distribution of c-fos oncogene expression in rats central nervous system following tiletamine anesthesia.Tiletamine action sites in CNS were studied,and general anesthetic mechanisms were also discussed.72 SD rats were randomly divided into 8 groups.At 0,10,30,60,90,100,120 and 180 min after given tiletamine 50 mg·kg-1 intraperitoneally,rats were perfused with 4% Paraformaldehyde(0.1 mol·L-1,PB,pH 7.4).The whole brain was separated into cerebral cortex,hippocampus,thalamus,cerebellum and brain stem.Then these brain tissues were dehydrated 24 h by 20% sucrose(4 ℃).Cryosections were 10 μm and Elivision Immunohistochemical technique for Fos-protein was used to observe and count on the Fos-like immunoreactive neurons.In normal saline group,there was few Fos-like immunoreactive neurons appeared.And Fos-like immunoreactive neurons were observed in cerebral cortex,hippocampus,thalamus,cerebellum and brain stem in experimental groups.Fos-like immunoreactive neurons expression increased at 10 min after tiletamine administrated by intraperitoneal injection,peaked at 60 min and decreased at 90 min.At 180 min after injection,Fos-like immunoreactive neurons recovered to base level,which had no significant difference compared with that before given tiletamine(P0.05).The results showed that tiletamine induce expression of c-fos oncogene in the rats′ cerebral cortex,hippocampus,thalamus,cerebellum and brain stem.And the cerebral cortex,hippocampus,thalamus,cerebellum and brain stem at the action sites of tiletamine.