Study of the diagnostic ultrasound causing fetal rat brain FOS expressing

Objective To evaluate whether diagnostic ultrasound could stimulate fetal central nervous system of rats.Methods The diagnostic ultrasound ( 5.0 MHz) was used to irradicatie pregnant wistar rats,which were divided into 30 min,20 min,10 min and control groups.The fetal brain tissues were collected.The FOS postive neurons were observed by using immunohischemistry and the analysis of variance was used to compare the difference groups.Results There was in a crowd of FOS postive neurons in the fetal brain hippocampus in the 30 min group and scattered poor FOS postive neurons in the 20 min and 10 min groups.No FOS postive neuron was found in the control group.There were significantly differences (P 0.001 ) between 30 min and other groups,but there was no significantly difference between 20 min and 10 min,20 min and control,10 min and control (P 0.05 ).Conclusions When the pregnant rats were irradiated for 30 minutes,the expression of FOS protein and c fos gene expressing substance in felal hippocampus neurons was increased indicating that the fetal rats central neurons might be stimulated.