Method study on residue analysis of florfenicol in aquatic products

In the paper, analytical method for determining florfenicol in aquatic products by using high performance liquid chromatography (HPLC) and using chloramphenicol as internal standards was reported. Florfenicol in fish samples are extracted with ethyl acetate, blowtryed and concentrated by N_2,purified with liquid-liquid separation, and determined by HPLC. The results of analysis indicated that the detection limit for florfenicol residue in aquatic products was 0.05 mg/kg and its recovery rates ranged from 95% to 101%. The relative standard deviation was less than 5% (n=5).