Molecular Marker Systems for DNA Fingerprinting and Diversity Analysis of Rice Germplasm

Rice breeding programs require a wide gene pool from which outstanding gene combinations can be assembled into a new variety.Germplasm diversity can be accurately evaluated through DNA fingerprinting.In this study,genetic variability and relationships among 265 rice germplasm accessions from the PhilRice Genebank were established from DNA fingerprints generated using two resistance gene analogue(RGA) markers and two Uniprimers:Seoulin Research Institute Life Science(SRILS).The DNA fingerprints showed that,on the average,the RGA and Uniprimer markers showed similar power in detecting polymorphism within the population for the entire germplasm population level.Seven dendrograms were generated based on banding profiles generated by single primers and their combinations featuring two nearly even major groups.Dendrogram analysis showed that both RGA and SRILS primers distinguished all 265 accessions,and revealed a high level of diversity among the accessions.The clustering patterns and the compositions of the groups were remarkably similar among the seven dendrograms ranging 88%~97% match.The two DNA marker systems studied could be ideal tools for rapid DNA fingerprinting and diversity analysis of rice germplasm varieties,and hence,can be an important adjunct in rice germplasm management such as in germplasm identification,diversity analysis and assembly of core collections.