Expression and identification of hepatocyte growth factor (HGF) gene in human umbilical vein endothelial cells (ECV304) with liposome in vitro

Objective To investigate hepatocyte growth factor (HGF) gene transfection and expression in human umbilical vein endothelial cells( ECV304) with liposome in vitro. Methods The recombinaint plasmid was constructed and transfected into human umbilical vein endothelial cells with liposome:GeneSHUTTLE-20, and positive clones were selectited by G418. The transfection and the expression of HGF in ECV304 were tested by RT-PCR and immunohistochemistry. Results After G418 selection, the cell clones were obtained successfully. Under a fluorescent microscope, the expression of enhanced green fluorescent protein(EGFP) was seen in cells transfected with vector contain EGFP gene. RT-PCR and immunohistochemistry proved that there was transcription of HGF gene in transfection cells and there was expression of HGF in these cells. Conclution The recombinant of pEGFP-N1-HGF is an effective expression vector. The recombinant of pEGFP- HGF transcript and express in the transfected cells. HGF is thus a potent agent for strategies designed to promote therapeutic angiogenesis.