Experimental study of hepatocyte growth factor recombinant gene expression in LO_2 cell line

Objective To examine the expression of hepatocyte growth factor in the LO 2 cell transfected with constructed expression plasmid containing the HGF cDNA.Methods The recombined eukarvotic vector, inserted with hepatocyte growth factor cDNA,PEGFP C1 HGF,transfected the culture cell LO 2 hepatocyte with lipofectamine.The inserted DNA was identified by PCR.The expression of hepatocyte growth factor protein was determined by measuring green fluorescent protein.Results (1) after four days of transfection with recombined PEGFP C1 HGF,the green fluorescent protein can be observed in the L0 2 cell.(2) the result of PCR showed the group transfected with PEGFP C1 HGF was characterized with the 306bp fragment,same size as the positive control,however,the group of pesu transfection and negative group had no character fragment.Conclusion Human eukarvotic expression vector,PEGFP C1 HGF,containing green fluorescent protein gene can successfully transfect LO 2 cell and express in it.