2004•Xibei Nong-Lin Keji Daxue xuebao. Ziran kexue banRequires access

PCR-RFLP molecular marker of pig H-FABP gene

Pang WeiJun

Abstract

In this experiment,genetic variations of 5′-upstream region and the second intron in 265 pig H-FABP gene including Duroc,Landrance,Largewhite,Neijiang pig,Rongchang pig,Hanjiang Black pig,Hanzhong White pig,Bamei pig and wild pig were checked by PCR-RFLP Molecular Marker with HinfⅠ,HaeⅢ and MspⅠ.The result showed that for HinfⅠ-RFLP,the above pig breeds had polymorphism and frequencies of allele H are 0.750 0,0.718 8,0.916 7,0.333 3,0.125 0,0.690 9,0.116 7,0.850 0 and (0.937 5) respectively;expect for Hanjiang Black pig(P0.05) and wild pig (P0.01),frequencies of gene and genotype in other breeds are in equilibrium of Hardy-Weinberg (P0.05);largewhite,Bamei pig,Hanjiang Black pig,Hanzhong White pig and wild pig present low polymorphism (PIC0.25) while other breeds have mediat polymorphism(0.25PIC0.5).Among tested breeds,only 4 Chinese local pig breeds have no polymorphism at the HaeⅢ-RFLP and MspⅠ-RFLP sites.

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Key concepts: Restriction fragment length polymorphism, Miniature pig, Biology, Genotype, Large white, Molecular biology, Domestic pig, Polymorphism (computer science)