Effect of KDR Gene Silence on Proliferation and Apoptosis of Gastric Cancer Cell Line MGC-803

[Purpose]To explore the effect of KDR gene in the proliferation and apoptosis of gastric cancer cell MGC-803.[Methods]The siRNA of KDR was constructed and transfected into MGC-803 cells with LipofectamineTM 2000.The expression of KDR mRNA and protein were detected by RTPCR and Western Blot method.Flow cytometry was used to detect the cell cycle,the cell proliferation was assessed by WST-1 assay,and cell apoptosis was detected by TUNEL.[Results]Compared with the control group and blank group,the mRNA and protein level was significantly decreased in the observation group when transfected with KDR siRNA.The growth slowed down and the cell cycle was arrested at G0/G1 phase and cell number in S phase was decreased in MGC-803 cell line(P0.05).After KDR siRNA transfection,the proliferation of MGC-803 cells was markedly inhibited and the apoptosis of MGC-803 cells was increased(P0.05).[Conclusion]Interference of KDR gene may suppress cell growth and promote cell apoptosis in MGC-803 cell line.Thus,KDR siRNA might be an effective target spot in the treatment for gastric cancer.