Protective effects of Dicaffeoylguinic Acid against CCl_4-induced injury in primary cultured rat hepatocytes ①

Aim To study the effects of dicaffeoylguinic acid on carbon tetrachloride (CCl 4) induced primary cultured rat hepatocytes.Methods Rat hepatocytes were isolated and injured by CCl 4.The proliferation and the viability of the hepatocytes were assayed by measuring the incoporating 3 H TdR and 3 H Pro respectively.The apoptosis and cell cycle of hepatocyte were determined by flow cytometry (FCM).And the activity of AST was examined.Results Dicaffeoylguinic acid 7.8～250mg·L -1 had no toxicity to the hepatocytes. The compound markedly increased the proliferation and the viability of the normal and injured rat hepatocyte,decreased AST activity in the cultural medium; restrained the hepatocytes apoptosis by CCl 4 and changed the cell cycle of hepatocytes.Conclusion Dicaffeoylguinic acid has notable anti-hepatocytes-injury effects in vitro.