2005•Chinese Journal of ProsthodonticsRequires access
Hypoxia affects the osteoblasts proliferation and apoptosis
Zhang Li-ha, Pla Gener
Open publisher page 0 citations
Abstract
Objective: To investigate the effects of hypoxia on osteoblasts pr oliferation and apoptosis. Methods: The osteoblasts obtained from the calvaria o f neonatal SD rats were cultured by method of enzyme digestion in vitro. Osteobl asts were counted by trypan blue staining on the 1st, 3rd, 5th, and 7th day afte r culture and the rate of apoptosis was detected by flow cytometer in normal env ironment and on the 1st, 3rd, and 5th day after cultured in hypoxic environment. Results: The cell number of hypoxic group was fewer than that of control group ; the rate of apoptosis increased gradually when the cells were cultured in norm al environment and hypoxic environment for 1?3?5 days. Conclusions: Hypoxia can inhibit the proliferation of osteoblasts and it can also facilitate the apoptosi s of osteoblasts.