Effect of fluoxetine on the mRNA expression of glia cell line-derived neurotrophic factor in the rat hippocampus of depression model

Objective To explore the effect of antidepressant drug(fluoxetine) on the mRNA expression of glia cell line-derived neurotrophic factor(GDNF) in the rat hippocampus of depression model.Methods Male Sprague-Dawley rats were divided into control group,control+fluoxetine group,depression model group and depression model+fluoxetine group.The depression model of rat was established by stimulation of separation and chronic mild unpredicted stress.The behavior was measured by open-field and sucrose water consumption test.The mRNA expression of GDNF in the rat hippocampus was assayed by Real-Time Reverse-Transcription PCR.Results The mRNA expression of GDNF in the rat hippocampus was significantly different in the four groups on the 21st day after the beginning of the stress(F=7.47,P0.01),the mRNA expression of GDNF in the rat hippocampus of the depression model group and the depression model+fluoxetine group were significantly higher than that of the control group and the control+fluoxetine group(P0.01 or P0.05).Conclusion The stress may increase the mRNA expression of GDNF in the rat hippocampus in a feed-back regulation mode.The fluoxetine has no effect on the mRNA expression of GDNF in the rat hippocampus.