THE EFFECT OF GLUCOSE ON EXPRESSION OF c-myc AND p21 GENE IN HUMAN PERITONEAL MESOTHELIAL CELLS

Objective:To investigate the effect of glucose on expression of c-myc and P21 gene in human peritoneal mesothelial cells.Methods:The cultured human mesothelial cells were exposed to culture medium containing different concentrations of glucose (1.5%,2.5%,4.25%),4.25% mannitol and normal culture medium as control for 24 hours,then mRNA expression was measured by semi-quantitative reverse-transcript PCR.Results:The level of c-myc mRNA and P21 mRNA expression was significantly higher in 2.5% glucose group and 4.25% glucose group than in 1.5% glucose group,4.25% mannitol group and normal culture medium group (P0.05).But there was no significant difference between the 1.5% glucose group,4.25% mannitol group and normal culture medium group(P0.05).The level of c-myc and P21 mRNA expression incresed as glucose concentration increased(r=0.968,0.967,P0.01).Conclusion:High glucose can up-regulate c-myc and P21 gene expression in a concentration-dependent manner.