Effect of Ginkgoflavones against brain edema and neuron ultrastructural injury on subarachnoid hemorrhage rats

Objective To determine the effect of Ginkgoflavones on brain water content and neuronic ultrastructure secondary to subarachnoid hemorrhage(SAH).Methods 80 mature male Wister rats were randomly divided into six groups:negatived control group,SAH group,SAH+normal saline(NS) group,SAH+50 mg/kg Ginkgoflavones group(Gf50)and SAH+200 mg/kg Ginkgoflavones group(Gf200).Cistern magna injection twice of freshly autologous arterial blood was used to induce SAH in rats.24 h and 72 h after induction of SAH,brain water content was detected.Electron microscope was used to observe neuronal ultrastructure 72h after SAH model inducted.The expressions of FasL protein in cerebral tissue were determined by immunohistochemistry.Results SAH increased brain water content.Compared with SAH group,brain water content in Ginkgoflavones groups were decreased;especially in Ginkgoflavones 200 mg/kg groups(P0.01),brain water content were a remarkable drop.The neuronal ultrastructures of the rats except negatived control group were different extent destruction.The destruction of neuronal ultrastructures were more severe in SAH group.neuronal ultrastructures in Ginkgoflavones groups were better than one of in SAH group.The expression of FasL protein in cerebral of SAH rats were significantly higher than normal control(P0.01),that of ginkgolides groups were lower than that of SAH groups,ginkgolides high dose groups were sigmificantly decreased(P0.05).Conclusions Ginkgolides may relieve brain edema and injury of neuronal ultrastructure in SAH rats.Ginkgolides can inhibit expression of FasL protein in SAH rat,especially in high dose group.