Study on the antagonistic activity of 6 strains of actinomycetes against Phytophthora capsici in plate
Niu Xiao-lei
Abstract
Niu Xiao-lei
Abstract
The paper studied the antagonism of 6 strains of actinomycetes against two kinds of Phytophthora capsici in different cultural media,cultural times and ultraviolet radiation treatments by agar lump method.The results showed that:The antagonism of 6 strains of actinomycetes had close relationship with their cultural media.Antagonistic activity was best in the PDA cultural medium,which can be used for the selecting of the actinomycetes against Phytophthora capsici.The antagonism of 6 strains of actinomycetes had close relationship to their cultural time.Using the agar lump method for the selecting of the antagonistic actinomycetes against Phytophthora capsici,the actinomycetes should be cultivated for 13 days.The 6 strains of actinomycetes had stronger antagonistic activity against Phytophthora capsici isolated from Qishan than that from Qinghai.The method of ultraviolet radiation irradiating the agar lump didn't reflect the structure of antibiotics secreted by strains whether it is stable or not.The product extracted from the fermentation liquids of the strains can reflect the structure of antibiotics whether it is stable or not.
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The paper studied the antagonism of 6 strains of actinomycetes against two kinds of Phytophthora capsici in different cultural media,cultural times and ultraviolet radiation treatments by agar lump method.The results showed that:The antagonism of 6 strains of actinomycetes had close relationship with their cultural media.Antagonistic activity was best in the PDA cultural medium,which can be used for the selecting of the actinomycetes against Phytophthora capsici.The antagonism of 6 strains of actinomycetes had close relationship to their cultural time.Using the agar lump method for the selecting of the antagonistic actinomycetes against Phytophthora capsici,the actinomycetes should be cultivated for 13 days.The 6 strains of actinomycetes had stronger antagonistic activity against Phytophthora capsici isolated from Qishan than that from Qinghai.The method of ultraviolet radiation irradiating the agar lump didn't reflect the structure of antibiotics secreted by strains whether it is stable or not.The product extracted from the fermentation liquids of the strains can reflect the structure of antibiotics whether it is stable or not.
Key concepts: Phytophthora capsici, Antagonism, Biology, Agar, Agar plate, Phytophthora, Botany, Microbiology