Screening,identification and biocontrol effect determination of antagonistic bacteria against Phytophthora capsici

More than 10 000 bacterial strains were separated from rhizosphere soil of vegetables from Tianjin,Hebei,Shandong,Shanxi,Fujian,Henan and Jiangsu,from which 802 strains exhibiting obvious antagonistic effect on Phytophthora capsici were selected.We further screened 62 strains having better antagonistic effect on Rhizoctonia solani,Botrytis cinerea and Pythium aphanidermatum from these 802 strains.Greenhouse pot experiments against P.capsici show that control effects of 6 strains are higher than 70%,Control effects of strain TB1340,82%,are highest.Determination results of its biology characteristics demonstrate that it has stronger activity producing chitinase,cellulase and β-1,3-glucanase.It is preliminarily identified as Bacillus sp.by 16S rRNA sequence analysis combined with morphology observation.