Expression of P16,Ki67 and HPV16/18 in cervical lesions and their clinical implications

Objective: To explore the expression of multiple tumor suppressor gene P16,proliferating cell nuclear antigen Ki67,and high risk human papilloma virus(HPV16/18) in cervical lesions and their clinical implications.Methods: Iimmunohistochemistry was used to examined the expression of P16 and Ki67 in cervical inflammatory lesions,cervical intraepithelial neoplasia(CIN)Ⅰ,Ⅱ,Ⅲand squamous cell carcinoma.HPV16/18 was detected by hybridization in situ.Results: P16 expression was confirmed in 20.0%,55.6%,93.6%,and100.0% of inflammatory lesions,CINⅠ,Ⅱ,Ⅲand squamous cell carcinoma,respectively.Positive expression of Ki67 was observed in 17.6%,61.1%,89.4%,and 100.0% of inflammatory lesions,CINⅠ,Ⅱ,Ⅲand squamous cell carcinoma,respectively.There were significant differences in P16 and Ki67 among the various groups(P0.05).The staining intensities of P16 and Ki67 increased with the severity of diseased cervical tissues,as well as their presence in the different epithelium layers.HPV16/18 was detected in 30.0%,61.1%,82.9%,and 100.0% of inflammatory lesions,CINⅠ,Ⅱ,Ⅲand squamous cell carcinoma,respectively.HPV was more prevalent in cervical lesions than that in inflammatory lesions.Conclusion: P16 and Ki67 proteins are useful as biological markers of cervical lesions;combined with HPV16/18 DNA detection,they can be helpful in screening and diagnosing cervical cancer and precancerous lesions.