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Effect of TAK1 siRNA on TAK1 Gene Expression in C2C12 Cells

Chen Dai-wen

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Abstract

Objective: To obtain the highly effective TAK1 siRNA.Method: Mouse myoblast C2C12 cells were transfected with the synthesized TAK1-specific small interfering RNA(siRNA) duplexes,siRNA ID# 94455,siRNA ID# 94549,and siRNA ID# 189006,respectively,using Lipofectamine 2000.The TAK1 mRNA abundance was determined using real-time quantitative PCR.Result: The expression levels of TAK1 mRNA in the cells transfected with siRNA ID# 94455,ID# 94549,and ID# 189006 were decreased 33.34%,46.73%,and 79.97%,respectively,compared to the negative control siRNA.Conclusion:The siRNA which can effectively down-regulate TAK1 gene expression was obtained in this study.

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What this paper is about

Objective: To obtain the highly effective TAK1 siRNA.Method: Mouse myoblast C2C12 cells were transfected with the synthesized TAK1-specific small interfering RNA(siRNA) duplexes,siRNA ID# 94455,siRNA ID# 94549,and siRNA ID# 189006,respectively,using Lipofectamine 2000.The TAK1 mRNA abundance was determined using real-time quantitative PCR.Result: The expression levels of TAK1 mRNA in the cells transfected with siRNA ID# 94455,ID# 94549,and ID# 189006 were decreased 33.34%,46.73%,and 79.97%,respectively,compared to the negative control siRNA.Conclusion:The siRNA which can effectively down-regulate TAK1 gene expression was obtained in this study.

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Available abstract

Objective: To obtain the highly effective TAK1 siRNA.Method: Mouse myoblast C2C12 cells were transfected with the synthesized TAK1-specific small interfering RNA(siRNA) duplexes,siRNA ID# 94455,siRNA ID# 94549,and siRNA ID# 189006,respectively,using Lipofectamine 2000.The TAK1 mRNA abundance was determined using real-time quantitative PCR.Result: The expression levels of TAK1 mRNA in the cells transfected with siRNA ID# 94455,ID# 94549,and ID# 189006 were decreased 33.34%,46.73%,and 79.97%,respectively,compared to the negative control siRNA.Conclusion:The siRNA which can effectively down-regulate TAK1 gene expression was obtained in this study.

Key concepts: Lipofectamine, Small interfering RNA, Transfection, C2C12, Gene silencing, Molecular biology, RNA interference, Messenger RNA

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Effect of TAK1 siRNA on TAK1 Gene Expression in C2C12 Cells — Research Paper | ScholarLens