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2012•The Chinese Journal of Modern Applied PharmacyRequires access

Selection of Specific siRNA Inhibiting the Expression of COX-2

TU Jiashenga

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Abstract

OBJECTIVE To select the siRNA which could most effectively inhibit the expression of COX-2,and to observe the change of COX-2 expression in cells.METHODS Three siRNAs were designed(siRNA24,siRNA954,siRNA1553) and one FAM fluorescence-labeled siRNA was used as a negative control(HK).Detect the effect of inhibition by RT-PCR and Western blot after transfecting human gastric cancer cell line SGC-7901.RESULTS The 50 μmol·L 1 Lipofectamine2000 had highest transfection effiency.The expression of human COX-2 gene was suppressed specific and effectively by siRNA954,compared with other groups(P0.01).CONCLUSION siRNA954 can inhibit COX-2 expression effectively.It plays an important role in the treatment of tumor.

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What this paper is about

OBJECTIVE To select the siRNA which could most effectively inhibit the expression of COX-2,and to observe the change of COX-2 expression in cells.METHODS Three siRNAs were designed(siRNA24,siRNA954,siRNA1553) and one FAM fluorescence-labeled siRNA was used as a negative control(HK).Detect the effect of inhibition by RT-PCR and Western blot after transfecting human gastric cancer cell line SGC-7901.RESULTS The 50 μmol·L 1 Lipofectamine2000 had highest transfection effiency.The expression of human COX-2 gene was suppressed specific and effectively by siRNA954,compared with other groups(P0.01).CONCLUSION siRNA954 can inhibit COX-2 expression effectively.It plays an important role in the treatment of tumor.

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Available abstract

OBJECTIVE To select the siRNA which could most effectively inhibit the expression of COX-2,and to observe the change of COX-2 expression in cells.METHODS Three siRNAs were designed(siRNA24,siRNA954,siRNA1553) and one FAM fluorescence-labeled siRNA was used as a negative control(HK).Detect the effect of inhibition by RT-PCR and Western blot after transfecting human gastric cancer cell line SGC-7901.RESULTS The 50 μmol·L 1 Lipofectamine2000 had highest transfection effiency.The expression of human COX-2 gene was suppressed specific and effectively by siRNA954,compared with other groups(P0.01).CONCLUSION siRNA954 can inhibit COX-2 expression effectively.It plays an important role in the treatment of tumor.

Key concepts: Transfection, Small interfering RNA, Western blot, Molecular biology, Cell culture, RNA interference, Gene, Gene expression

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