TO DEMONSTRATE MICROVASCULATURE OF SPINAL CORD AND CELLS OF NEROUS TISSUE CONCURRENTLY BY IMPROVED TA-FE METHOD

Objective: To observe the effect of improved TA-Fe method to demonstrate microvascular and cells of nervous tissue in spinal cord concurrently under light microscopy. Methods: The microvascular in spinal cord were stained with TA-Fe method, then cells of nervous tissue re-dyed by Harris hematlxylin or hematoxylin-eosin (HE) methods. Results: All microvascular and cells in spinal cord were well displayed. The density of microvascular varies from position to position, in the areas which the neurons is density distributed, the microvascular is also density, vice versa. Conclusion: Microvascular and cells of nervous tissue in spinal cord were demonstrated concurrently by improved TA-Fe method, relation between cells and vessel were shown. It is a useful new method to study blood-deficiency diseases of central nervous system.