Spectrophotometric Method for Determination of Aciclovir with Bromocresol Purple and Bromocresol Green

A sensitive spectrophotometric method is developed for determination of aciclovir.In pH 3.30 NH2CH2COOH-NaCl-HCl buffer medium,aciclovir(ACV) reacts with bromocresol purple(BCP) or bromocresol green(BCG) to form an ion-association complex,the colours of solutions change obviously,the maximum fading wavelengths are at 428 nm(BCP-ACV) and 450 nm(BCG-ACV),respectively,and the fading intensity is proportional to the concentration of aciclovir.For BCP-ACV and BCG-ACV systems,the Beer's law are obeyed in the concentration range of 0~2.69×10-5 mol/L and 0~2.58×10-5 mol/L,the apparent molar absorptivity are 2.53×104 L·mol-1·cm-1 and 1.79×104 L·mol-1·cm-1,and the detection limits of ACV are 5.10×10-7 mol/L and 7.13×10-7 mol/L,respectively.The method has high sensitivity and fairly good selectivity,and has been applied to the determination of aciclovir in medicine samples and human serum and human urine with satisfactory results.