Molecular Spectral Characters of Interaction of Pioglitazone Hydrolchloride with Some Acidic Triphenylmethane Dyes and Their Analytical Applications

In Britton-Robinson buffer media with different pH values,some acidic triphenylmethane dyes can form ion-association complexes with pioglitazone hydrolchloride respectively.The maximum fading wavelength of complexes is between 558 nm and 616 nm,and the maximum absorption wavelength is between 426 nm and 450 nm.The concentration of pioglitazone hydrolchloride follows Beer′s law for the tight absorption in a range of 3.1×10-7 mol/L and 1.55×10-5 mol/L,the apparent molar absorptivity is between 1.47×104 L/(mol·cm) and 9.50×104 L/(mol·cm),and the detection limit is between 3.32×10-5 g/L and 2.57×10-4 g/L respectively.A sensitive spectrophotometric method is therefore developed for the determination of pioglitazone hydrolchloride.The method has a high sensitivity and a fairly good selectivity,and has been applied to the detemfination of pioglitazone hydrolchloride in urine samples,mixture samples and blood samples,with satisfactory results.