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2007•Chinese Journal of HypertensionRequires access

C-Reactive Protein Enhances the Expression of Matrix Metalloproteinase-2 in Human Umbilical Vein Endothelial Cells

XU Bia

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Abstract

Objective To investigate the effects of C-reactive protein(CRP)on expression of matrix metalloproteinase-2(MMP-2)in human umbilical vein endothelial cells(HUVEC).Methods HUVEC were cocultured with different concentration of CRP for different time.MMP-2 protein and mRNA expression in HUVEC were measured by Western blotting and reverse transcription polymerase chain reaction(RT-PCR).Results Incubation of HUVEC with CRP results in an increasing expression of protein and mRNA of MMP-2 in a concentration and time-depedent manner.Conclusion CRP enhances the expression of MMP-2 in HUVEC.

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What this paper is about

Objective To investigate the effects of C-reactive protein(CRP)on expression of matrix metalloproteinase-2(MMP-2)in human umbilical vein endothelial cells(HUVEC).Methods HUVEC were cocultured with different concentration of CRP for different time.MMP-2 protein and mRNA expression in HUVEC were measured by Western blotting and reverse transcription polymerase chain reaction(RT-PCR).Results Incubation of HUVEC with CRP results in an increasing expression of protein and mRNA of MMP-2 in a concentration and time-depedent manner.Conclusion CRP enhances the expression of MMP-2 in HUVEC.

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Available abstract

Objective To investigate the effects of C-reactive protein(CRP)on expression of matrix metalloproteinase-2(MMP-2)in human umbilical vein endothelial cells(HUVEC).Methods HUVEC were cocultured with different concentration of CRP for different time.MMP-2 protein and mRNA expression in HUVEC were measured by Western blotting and reverse transcription polymerase chain reaction(RT-PCR).Results Incubation of HUVEC with CRP results in an increasing expression of protein and mRNA of MMP-2 in a concentration and time-depedent manner.Conclusion CRP enhances the expression of MMP-2 in HUVEC.

Key concepts: Umbilical vein, Matrix metalloproteinase, Messenger RNA, Molecular biology, Reverse transcription polymerase chain reaction, Blot, Protein expression, Real-time polymerase chain reaction

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