The Factors Influencing the High-level Expression of Recombinant Protein in CHO Cells

It is very significant to produce recombinant protein in biopharmaceutical.Chinese hamster ovary cells (CHO cells) have become the best eukaryotic expression systems for glycoproteins.The factors influencing the high level expression of recombinant protein in CHO cells were discussed,including CHO cell expression systems,vector systems,recombinant gene,the screen and amplification of expression clone,the large scale culture of recombinant cells.It is helpful to apply different influencing factors flexibly for the high level expression of recombinant protein in CHO cells.The questions of CHO expression mainly focus on high level expression,isolation,purification,production equipment and production cost.With the improvement of these problems,CHO cell expression system will satisfied the demands of biopharmaceutical better.