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2005•Journal of Shandong UnivenityRequires access

Inhibitory effect of chanfuning serum on the apoptosis of PC12 cells induced by 6-hydroxydopamine

Baohua Cheng

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Abstract

Objective: To study the inhibitory effect of chanfuning serum on the apoptosis of PC12 cells induced by 6-hydroxydopamine (6-OHDA). Methods: Serum pharmacological method was used to make chanfuning serum. 6-OHDA and chanfuning serum were added into the medium of PC12 cells separately. MTT method was used to assay the cell's viability, and tyrosine hydroxylase (TH) and caspase-3 immunocytochemistry staining were used to evaluate the content of TH and count the number of apoptosis cells. Results: PC12 cell's viability and calibrated optic density of TH positive cells enhanced (P0.01), and the number of caspase-3 positive cells decreased(P0.01) significantly in chanfuning serum treated group compared with 6-OHDA treated group. Conclusion: Chanfuning serum may have direct nutritious effect, protect PC12 cells from injury induced by 6-OHDA, and produce anti-apoptosis effect to some extent.

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What this paper is about

Objective: To study the inhibitory effect of chanfuning serum on the apoptosis of PC12 cells induced by 6-hydroxydopamine (6-OHDA). Methods: Serum pharmacological method was used to make chanfuning serum. 6-OHDA and chanfuning serum were added into the medium of PC12 cells separately. MTT method was used to assay the cell's viability, and tyrosine hydroxylase (TH) and caspase-3 immunocytochemistry staining were used to evaluate the content of TH and count the number of apoptosis cells. Results: PC12 cell's viability and calibrated optic density of TH positive cells enhanced (P0.01), and the number of caspase-3 positive cells decreased(P0.01) significantly in chanfuning serum treated group compared with 6-OHDA treated group. Conclusion: Chanfuning serum may have direct nutritious effect, protect PC12 cells from injury induced by 6-OHDA, and produce anti-apoptosis effect to some extent.

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Available abstract

Objective: To study the inhibitory effect of chanfuning serum on the apoptosis of PC12 cells induced by 6-hydroxydopamine (6-OHDA). Methods: Serum pharmacological method was used to make chanfuning serum. 6-OHDA and chanfuning serum were added into the medium of PC12 cells separately. MTT method was used to assay the cell's viability, and tyrosine hydroxylase (TH) and caspase-3 immunocytochemistry staining were used to evaluate the content of TH and count the number of apoptosis cells. Results: PC12 cell's viability and calibrated optic density of TH positive cells enhanced (P0.01), and the number of caspase-3 positive cells decreased(P0.01) significantly in chanfuning serum treated group compared with 6-OHDA treated group. Conclusion: Chanfuning serum may have direct nutritious effect, protect PC12 cells from injury induced by 6-OHDA, and produce anti-apoptosis effect to some extent.

Key concepts: Hydroxydopamine, Apoptosis, Viability assay, MTT assay, Immunocytochemistry, Tyrosine hydroxylase, Inhibitory postsynaptic potential, Chemistry

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