Effects of Qizhi Jiangtang Capsule on Vascular Endothelial Growth Factor and Extracellular Matrix of Kidney in Diabetic Nephropathy Rats

Objective: To Study effects of Qizhi Jiangtang Capsule( QJC) on the expressions of VEGF and ECM in kidney tissues of DN rats. Methods: 40 of the 48 male Wistar rats( 6 to 7 weeks) were randomly divided into 4 groups: the sham operation group,the model group,the Valsartan group( control group),the low dosage of QJC granules group( low dosage group) and high dosage of QJC granules group( high dosage group),and each group with 10 rats. Except the rats of the sham operation group,the other rats were established DN animal model by removaling the right kidney with intraperitoneal injection of STZ. Medicine was given to each group according to the designed concentration and dosage after 2 days of the model established. Serum creatinine. blood urea nitrogen,cystatin- C,β2- microglobulin,24 hour surinary protein quantitative,urine microalbumin and UmALB /Ucr of DN rats were tested and recorded every 4 weeks. All animals were put to death after being treated 8 weeks. Histological change in the kidney tissues was examined by HE stain. Kidney tissue was observed under optical microscope. Kidney podocyte was observed under electron microscope. VEGF,Matrix Metalloproteinase 9( MMP- 9),Matrix Metalloproteinases Inhibitory Factor1( TIMP- 1),fibronectin( FN) andⅣ Type Collagen( ColⅣ) were detected by immunohistochemical. Results: At all time points,Scr,BUN,Cystatin- C,β2- MG,24 h TP,mALB and UmALB /Ucr of the model group were higher than those of the sham operation group( P 0. 05),the control group was lower than the model group( P 0. 05),the QJC groups were higher than the sham operation group with dose dependent( P 0. 05 ~ 0. 01). Compared with the model group and control group,HE staining showed that kidney and vessel pathological damage of DN rats were lessened by QJC. Observations of electron microscopy show that kidney thickening of basement membrane,endothelial cell and mesangial proliferation can obviously be relieved by QJC,podocyte foot process fusion also can be reduced with dose dependent. Immunohistochemical showed that: Expressions of VEGF,TIMP- 1,FN,Col Ⅳ in model group have increased more significantly than that of the sham operation group( P 0. 05). Comparing these indexes,the control group and the low dosage group were significantly lower than the model group( P 0. 05),the high dosage group was significantly lower than the low dosage group( P 0. 01). MMP- 9 express the most obvious in the sham operation group,the model group was significantly lower than the sham operation group( P 0. 01). The control group and the low dosage group were higher than the model group,the high dosage group was significantly higher than the control group and the low dosage group( P 0. 05 ~ 0. 01). Conclusion: The excessive synthesis and deposition of VEGF in the kidney tissues can be obviously inhibited,and the ECM expression in kidney tissue can be effectively controlled by QJC,thus the DN rat renal pathological damage tissue and vascular structures can be improved.