Effect of Qizhi Jiangtang capsule on function and structure of kidney in diabetic nephropathy rats

[Abstrac] Objective To study effect of Qizhi Jiangtang Capsule(QJC) on renal structure and function of kedney in DN rats. Methods 48 male Wistar rats were random Ly divided into 5 groups(n=8): the sham operation group,the model group,Valsartan group(control group), the low-dosage of QJC group(low dosage group) and high dosage of QJC group(high dosage group). Except the rats in the sham operation group,the other rats were established DN animal model by removal of the right kidney with intraperitoneal injection of STZ.Medicine was given to each group according to the designed concentration and dosage from 2 days of the model established. 24 h UPQ, α1-microglobulin(α1-MG),β2- microglobulin(β2-MG)and serum creatinine(Scr), Cystatin-C of DN rats were observed at 12 th week. Ang Ⅱ、ET-1 and NO were detected,too. The kidney tissues was stained respectively by HE,PAS and immunohistochemical.Histological change in the kidney tissues was observed by HE stain. GSI and IF were calculated by PAS stain.Glomerular capillary ultrastructure was observed by electron microscopy. The expression of TGF-β1 was detected by immunohistochemical. Results At 12 th week, compared with the sham group, 24 h UPQ、α1-MG、β2-MG of model group were significantly raised(P 0.01). These indicators of 3 treated groups rats were significantly reduced(P 0.01). Compared with the control group,the above contents of 2 groups of QJC were obviously decreased(P 0.05-0.01),and dose-dependent manner. BS、Scr、Cystatin-C of the model group were also significantly raised(P 0.01)at same time.To compared with the control group,these indicators of 2 groups of QJC were obviously decreased(P 0.05-0.01), BS of the control group was shown no significant change(P 0.05). To compared with the control group,Ang Ⅱ、ET-1 of 3 treated groups rats were significantly reduced(P 0.01), NO were significantly raised(P 0.01). HE staining showed that renal pathological damage were lessened by QJC. PAS stain showed that GSI、IF were significantly lessened in 2 traditional Chinese medicine treatment groups(P 0.01).Electron microscopy observations show that thickening of glomerular basement membrane and mesangium hyperplasia were reduced by QJC, podocyte foot process fusion reduced. Immunohistochemical tests show that the expression of renal tissue TGF-β1 were down-regulated by QJC(P 0.01). Conclusion Through secretion of Ang Ⅱ was suppressed, vascular endothelial damage reduced,the expression of TGF-β1 in the renal tissues were down-regulated, structure and function of kidney tissues improved, DN is treated by QJC.