Determination of major sugars and citric acid in raw orange juice were by Nuclear Magnetic Resonance at the same time

Various configurations of glucose,fructose,sucrose and citric acid in raw orange juice were determined by NMR when dissolved in heavy water.Glucose,fructose,sucrose and citric acid were attributed by fingerprint. Simulated spectra confirmed glucose,fructose and sucrose citric acid’s existence in raw orange juice.Potussium biphthalate was selected as an internal standard,1H-NMR spectra was used to determine the content of glucose, fructose and sucrose citric acid at the same time.RSD( n = 6) of glucose,fructose and sucrose citric acid were 3.61%,3.24%,2.19% and 4.6% respectively,recovery of them were 99.4%,98.8%,97.8% and 95.7%,respectively. This method used only a little sample and was simple,rapid,accurate and suitable for the determination of the contents of glucose,fructose,sucrose and citric acid in raw orange juice at the same time.