2000•Journa of Henan Medical UniversityRequires access
Isolation and purification of antineoplastic polypeptide III from APBMV
Kong Tianhan, Guo Jinwu, Xiao Guiyuan, Yang Zhuoping, Zhou Shaoxiong, Xue‐Fei Han, Huayan Chen, Weihua Dong
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Abstract
Aim: To explore the methods on isolation and purification of antineoplastic polypeptide Ⅲ from APBMV (AP Ⅲ). Methods: The AP Ⅲ was isolated from scorpion venom of Buthus martensii karsch by pretreatment, ion exchange chromatography (Sepharose FF, column:25 cm×5 cm,flow rate:0.8 ml/min,linear gradient:1 200 min) or coupling gel filtration(Sephadex G 50, column:100 cm×5 cm,flow rate:0.5 ml/min) with Sepharose FF chromatography. The purity of AP Ⅲ and other compositions of scorpion venom were inspected by high performance liquid chromatography. Results:The purification rate and output of AP Ⅲ were 89% and 2.24% with ion exchange chromatography only,but 95% and 4.72% with coupling Sephadex G 50 gel filtration with Sepharose FF chromatography.Conclusion:The fractionating method of coupling Sephadex G 50 gel filtration with Sepharose FF chromatography is useful for improving the purification rate and output of AP Ⅲ. The experimental result is thought as an important evidence to make clinical use of AP Ⅲ in treatment of tumor.