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2001•Journa of Henan Medical UniversityRequires access

Research on analysis of APBMV and its components

Kong Tianhan

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Abstract

Aim: To explore the methods on analysis and evaluation of antineoplastic polypeptide from Buthus martensii venom(APBMV) and its components. Methods: The components of APBMV were separated by ion exchange chromatography (Sepharose FF, column:25 cm×5 cm,flow rate:0.8 ml/min,linear gradient:1 200 min),and were inspected by high performance liquid chromatography(HPLC) and high performance capillary electrophoresis(HPCE). Results: APBMV could be separated into 2 components(component 1 and 2 ) by Sepharose FF ion exchange chromatography and 4 peaks(peakⅠ～Ⅳ) by HPLC. It was displayed by HPLC and HPCE that the peakⅠwas mainly made up from the component 1(purification rate: 86.35%) and the peak Ⅲ, the component 2 ( 69.57%). Conclusion: Sepharose FF chromatography is useful for improving the purification rate and output of components of APBMV. The quantity and purity of components from scorpion venom can be displayed obviously by HPLC and HPCE.

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What this paper is about

Aim: To explore the methods on analysis and evaluation of antineoplastic polypeptide from Buthus martensii venom(APBMV) and its components. Methods: The components of APBMV were separated by ion exchange chromatography (Sepharose FF, column:25 cm×5 cm,flow rate:0.8 ml/min,linear gradient:1 200 min),and were inspected by high performance liquid chromatography(HPLC) and high performance capillary electrophoresis(HPCE). Results: APBMV could be separated into 2 components(component 1 and 2 ) by Sepharose FF ion exchange chromatography and 4 peaks(peakⅠ～Ⅳ) by HPLC. It was displayed by HPLC and HPCE that the peakⅠwas mainly made up from the component 1(purification rate: 86.35%) and the peak Ⅲ, the component 2 ( 69.57%). Conclusion: Sepharose FF chromatography is useful for improving the purification rate and output of components of APBMV. The quantity and purity of components from scorpion venom can be displayed obviously by HPLC and HPCE.

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Available abstract

Aim: To explore the methods on analysis and evaluation of antineoplastic polypeptide from Buthus martensii venom(APBMV) and its components. Methods: The components of APBMV were separated by ion exchange chromatography (Sepharose FF, column:25 cm×5 cm,flow rate:0.8 ml/min,linear gradient:1 200 min),and were inspected by high performance liquid chromatography(HPLC) and high performance capillary electrophoresis(HPCE). Results: APBMV could be separated into 2 components(component 1 and 2 ) by Sepharose FF ion exchange chromatography and 4 peaks(peakⅠ～Ⅳ) by HPLC. It was displayed by HPLC and HPCE that the peakⅠwas mainly made up from the component 1(purification rate: 86.35%) and the peak Ⅲ, the component 2 ( 69.57%). Conclusion: Sepharose FF chromatography is useful for improving the purification rate and output of components of APBMV. The quantity and purity of components from scorpion venom can be displayed obviously by HPLC and HPCE.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Capillary electrophoresis, Sepharose, Ion chromatography, Enzyme, Biochemistry

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