Effects of autologous transplantation of bone marrow mesenchymal stem cells on heart function of ischemic myocardium in rabbits

Objective: To investigate the feasibility that bone marrow mesenchymal stem cells (MSCs) differentiate into cardiomyocytes in ischemic myocardium microenvironment and whether they improve heart function. Method: The left anterodecendant arteries (LAD) of adult rabbits were ligated so as to establish animal models of acute myocardial infarction. MSCs were isolated from bone marrow and were cultured in cell medium or medium with 5-azacytidine(5-aza, 10 μmol/L). 2 weeks later the expanded MSCs labeled with BrdU were injected into the scar tissue. The heart function of each rabbit was measured by echocardiography and hemodynamics. Specimens were taken 4 weeks after implantation. Histologic sections of left ventricular were stained by immunofluorescent and examined under fluorescent microscope. Result: The implanted cells were found in both groups and not in the control group. Compared with control group, 4 weeks after implantation two transplanted groups result in shorter LVESD ,LVED Dandh igherEF ,△D % ,±dp/d tmax an dlow erLVEDP .There is nosignifican cebetwee nthegroup with 5 azaan dthe groupwi thout 5 aza .Conclusion :A utologo usbonema rrowmesenchymalstemcellscandifferentiate intoc ardiomyo cytesinvivotore pairinf ractedmyoca rdi umandim prov ethehear tfunction .Bone marrowMS Cstrans plan tati oncanused to therapy acute myocardial infarction .