The Effect of Genistein on Mouse Erythroleukemia Cell Line

Mouse erythroleukemia cell line MEL was chosen as the target of genistein, to study its suppression effects, and the possible mechanisms. It was proved that genistein can greatly inhibit the growth and proliferation of MEL cell ( IC 50 is 7 12?mg/L) by use of MTT and growth curve. Flow cytometry analysis proved that Genistein can induce a G 2 M phase cell cycle arrest, but has little effect on apoptosis. Gel electrophoresis indicated that genistein can cause genomic DNA damage. Benzidine dying proved that genistein has a promising effect of inducing MEL to differentiate into erythoid cell in a time and dose dependent manner. RT PCR showed genistein can inhibit the c myc expression in MEL cell. The biorheology measurements indicated that cell surface charge density decreased, the osmotic fragility increased, and the cell maximum deformation decreased.