Effect of Lipid Deposition Related Gene Expression and Synthase Activity by Glucose in Cultured Goose Hepatocytes

The goal of this experiment was to investigate the effects of glucose on lipids deposition in primary hepatocytes of Tianfu meat goose(Anser cygnoides). The results indicated: 0~25 mmol/L glucose had no significant effect on the content of aspartate transaminase(AST) and alanine amiotransferase(ALT) in cell culture supernatant. Although 35 mmol/L glucose had an obvious effect, the concentrations of AST and ALT within normal range. That's to say, hepatocytes is fine. Furthermore, the results of Oil Red-O staining revealed that the glucose significantly promoted lipid droplet accumulation and lipid deposition. Compared with the control group, lipids accumulation, acetyl-CoA carboxylase α(ACCα) and fatty acid synthetase(FAS) activity were significantly increased by 25 and 35 mmol/L glucose. 5, 25, and 35 mmol/L glucose increased significantly the mRNA expression of ACCα and FAS. In conclusion, glucose could induce hepatic lipids deposition by up-regulating gene expression and enzyme activity of ACCα and FAS in goose hepatocytes.